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Penelitian

Prof.Dr.Indropo Agusni, dr.,Sp.KK (K)

indropo49@gmail.com

Fakultas Kedokteran / Departemen Kesehatan Kulit dan Kelamin

Tim Peneliti :

  1. Indropo Agusni
  2. Yoes Prijatna Dachlan
  3. Fauziah Nuraini Kurdi --> Peneliti Utama
  4. Indro Handojo

Tahun : 2010

Halaman Naskah : 6 halaman

Sumber Dana : mandiri

Besaran Dana : 0

SK. Penetapan : mandiri

Publikasi : Media Jurnal : The Southeast Asian J of Trop Med and Publ Health 41:5 (1158-1163) , 2010

Kategori Penelitian : Kesehatan

Posting : 04-09-2012

Visitor : 4445


Download Article : PDF 3955 byte.


Title :

AMINO ACID SEQUENCE OF B CELL EPITOPE OF N-TERMINAL REGION OF ESAT-6 MYCOBACTERIUM LEPRAE ROLE AS SPECIFIC ANTIGEN FOR DIAGNOSIS OF LEPROSY

Author : Prof.Dr.Indropo Agusni, dr.,Sp.KK (K)


Year : 2010

Abstact :

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AMINO ACID SEQUENCE OF B CELL EPITOPE OF

N-TERMINAL REGION OF ESAT-6 MYCOBACTERIUM

LEPRAE ROLE AS SPECIFIC ANTIGEN FOR DIAGNOSIS

OF LEPROSY

 

 

Fauziah Nuraini Kurdi, Indro Handojo, Indropo Agoesni and Yoes Priatna Dahlan

 

Physical and Health Study Program, Teacher Training and Education Faculty and

Rehabiltitation Medicine, Medical Faculty, Sriwijaya University, Palembang;

Department of Clinical Pathology, Department of Dermatology and Venerology,

Department of Parasitology, Medical Faculty, Airlangga University, Surabaya,

Indonesia

 

 

Abstract. The objective of this study was to find a specific B-cell epitope of Nterminal region of antigen L-ESAT-6 from leprosy patients, healthy individuals and healthy nurses working for more than 10 years in the leprosy ward of dr.A.Rivai Abdullah Leprosy Hospital, Palembang, Indonesia. Fifty subjects were enrolled in this study, comprising 10 subjects with LL type leprosy, 10 subjects with BB type leprosy , 10 subjects with TT type leprosy, 10 healthy nurses from leprosy ward and 10 healthy individuals as control group. The amino acid sequence of residues 11-36 of the N-terminal region of L-ESAT-6 were divided into a series of 18 peptides each consisting of 9-mer peptides with an overlap of 8-mers and an offset of one amino acid. The series of 18 peptides were synthesized in the form of biotinylated peptides and used to screen sera of 50 subjects using an indirect ELISA method. Our study identified at the N-terminal of L-ESAT-6, LEQCQES, VNELQG and IDALLE as epitope marker for LL and BB type of leprosy, epitope marker for TT type of leprosy and for protective epitope marker) healthy nurses working for more than 10 years in the leprosy ward, respectively. These antigens can be used in immunochromatographic test for the early diagnosis of leprosy.

 

Key words: L-ESAT-6, B-cell epitope mapping, M. leprae diagnostic tool, protective marker, leprosy marker

 

 

 


Keyword : : L-ESAT-6, B-cell epitope mapping, M. leprae diagnostic tool, protective marker, leprosy marker ,


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