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Penelitian

Dr. Cita Rosita Sigit Prakoeswa, dr.,Sp.KK(K)

citarositasp@yahoo.com

Fakultas Kedokteran / Departemen Kesehatan Kulit dan Kelamin

Tim Peneliti :

  1. Cita Rosita Sigit Prakoeswa --> Peneliti Utama
  2. Indropo Agusni
  3. Adri D Prasetyo, Ratna Wahyuni
  4. Iswahyudi
  5. Dinar Adriaty
  6. Shinzo Izumi

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Kategori Penelitian : Kesehatan

Posting : 07-03-2012

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Title :

Genotyping Analysis of Mycobacterium leprae in Water Environment of Endemic Places of Leprosy in Lamongan, East Java and Study of Transmission Pattern of Non-Human Source of Infection

Author : Dr. Cita Rosita Sigit Prakoeswa, dr.,Sp.KK(K)


Year : 0

Abstact :

Background Leprosy sources of infection are direct contact of human transmissions or indirectly through the environment (non-human transmissions). To study about non-human transmissions of M. leprae infection in endemic areas, it is important to detect the distribution of M. leprae in the environment and population using genotyping analysis.

Methods The study was conducted at the two endemic areas of leprosy. Samples were taken from water and soil sources, nasal swab from household contact, and speciment of skin tissue from active leprosy patient. Standardized methods of DNA extraction from samples, PCR for M. leprae detection, and PCR amplification for M. leprae genotyping were then performed, continued by genotyping analysis using TTC repeats variations.

Results Total of 109 samples were taken, consist of 24 skin speciment from leprosy patients, 49 nasal swab samples from patients and household contacts, 18 water samples and 18 soil samples. 27,78% water samples are positive of M. leprae which the water came from the natural sources TTC repeats showed that the strain from water is not matching with the strain from skin smear nor nasal swab of patients and household contacts.

Conclusion Genetic variations were found between M. leprae from patients and from environment. Further research is needed to prove the intimate relationship of host-agent-environment in the transmission of leprosy.

 

Key words: genotyping, M.leprae, environment, leprosy, transmission


Keyword : genotyping, M.leprae, environment, leprosy, transmission,


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