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Penelitian

Prof. Dr. Suhartono Taat Putra, dr., MS

taatputra@yahoo.com

Fakultas Kedokteran / Departemen Patologi Anatomi

Tim Peneliti :

  1. Subijanto Marto Sudarmo --> Peneliti Utama
  2. Suhartono Taat Putra
  3. Pitono Soeparto
  4. Marsetyawan Hne Soesatyo

Tahun : 1999

Halaman Naskah : 72 halaman

Sumber Dana :

Besaran Dana : 0

SK. Penetapan : SK Rektor Unair No. 8229/J03/PP/1999

Publikasi : Seminar : Pengaruh Defisiensi Vitamin A Terhadap Status Imun Dan Respons Imun Mukosa usus (Penelitian Eksperimental Pada Tikus Wistar Melalui Pendekatan Imunopatobiologik)

Kategori Penelitian : Kesehatan

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Title :

Pengaruh Defisiensi Vitamin A Terhadap Status Imun Dan Respons Imun Mukosa usus (Penelitian Eksperimental Pada Tikus Wistar Melalui Pendekatan Imunopatobiologik)

Author : Prof. Dr. Suhartono Taat Putra, dr., MS


Year : 1999

Abstact :

Diarrheal disease in infants and children remains a major health problem in the world, especially in developing countries including Indonesia, as is indicated by their high morbidity and mortality rate. Majority of diarrheal patients in developing countries are mostly accompanied by vitamin A deficiency, whether it is subclinically or manifested clinically.

Within 25 years various efforts have been attempted to prevent and eradicate diarrheal disease in infants and children. Numerous approaches, including rehidration, formula feeding, medication, hygiene and sanitation programs, which were not based on reliable concepts, have been tried without satisfying results.

It has been assumed that there are possibly other factors that may play an important role dealing with the high morbidity and mortality of diarrheal disease especially in developing countries. One of those factors proposed is the immune status of the mucosa of children suffering from diarrhea which will be the main concern of this study.

The objective of this study was to elucidate the immunopathobiogenesis of vitamin A deficiency in experimental animal models. The animals were divided into three groups, i.e., vitamin A deficient group, control group and vitamin A deficient treated group. Each group consisted of 20 male Wistar strain rat. To evoke mucosal immune response, they were exposed to orally-given LPS.

Immune response variables used in this study were: dendritic cell, NK cell, neutrophyl CD 11b, CD4  T cell, CD8 T cell, IL-2 producing T cell, IFNg producing T cell, IL-4 producing T cell, IL-10 producing T cell, IgA producing plasma cell, IgG producing plasma cell and IgM producing plasma cell.

The results of the study show that in efector sites vitamin A deficient group exposed to LPS showed significant decrease in mucosal immune response, compared to control group with Wilks value of 0.007. There was a significant improvement of mucosal immune response in vitamin A treated group, compared to vitamin A deficient group with Wilks value of 0.014. Comparison between control group and vitamin A treated group on mucosal immune response show no significant difference with Wilks value of 0.589. Without exposing to LPS, vitamin A deficient group shows declining mucosal immune status, compared to control group with Wilks value of 0.024. There was significant improvement of mucosal immunity status in vitamin A treated group compared to vitamin A deficient group (Wilks 0.011) and no significant difference compared to control group (Wilks 0.142).

The other results of the study show that in inductive sites vitamin A deficient group exposed to LPS showed significant decrease in mucosal immune response, compared to control group with Wilks value of 0.023. There was no significant improvement of mucosal immune response in vitamin A treated group, compared to vitamin A deficient group with Wilks value of 0.166. Comparison between control group and vitamin A treated group on mucosal immune response show no significant difference with Wilks value of 0.365. Without exposing to LPS, vitamin A deficient group shows no significant difference on mucosal immune status, compared to control group with Wilks value of 0.113. There was significant improvement of mucosal immune status in vitamin A treated group compared to vitamin A deficient group (Wilks 0.021) and no significant difference compared to control group (Wilks 0.259).

In addition, this study also able to construct six immunopathobiogenesis models of mucosal immune status and response. Exposing vitamin A deficient group to LPS will significantly suppress the local humoral and cellular immune responses. Treatment with vitamin A improves the mueosal immune system. unexposed to LPS, vitamin A deficient group showed abnormal cellular and local humoral immunity status. Based on the finding of this study, it is concluded that vitamin A can act as an immunomodulator for mucosal immune system.

The six models of immunopathobiogenesis related to the mucosal immune status and response to vitamin A deficiency can be used as a new basic concepts for eradicating and preventing diarrheal disease in infants and children, in view of vitmin A compaign and development of oral immunization.


Keyword : Vitamin A deficiency, stress immunocompetent cells, mucosal immune response, pathobiology, immunopathobiogenesis,


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